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蛋白质外聚物中多糖的比例——结论、致谢!

来源:上海谓载 浏览 11 次 发布时间:2021-10-12

四、结论


油和/或 Corexit 的存在会导致 EPS 的蛋白质:多糖比率更高,并在中胚层实验中降低 SFT。 在这些实验中,SFT 与 蛋白质:具有负斜率的 EPS 多糖。 当开阔的海洋 水域和两种不同的沿海水处理进行了比较, 蛋白质趋势:多糖为 CEWAF > DCEWAF > WAF ≥ Control 并且对于 SFT,它是相反的, CEWAF < DCEWAF < WAF ≤ 对照。 因此,SFT 与胶体 EPS 中的蛋白质:多糖比率成反比。


当中宇宙水柱的不同尺寸分数为 相比之下,我们发现 EPS 胶体可以降低 SFT 蛋白质:多糖比例,表明有效的生物乳化 蛋白质的容量。 粒子滤波中 SFT 的比较 分数 (< 0.45 μm) 和 EPS 胶体分数 (< 0.45 μm 和 > 3 kDa),对于真正溶解的部分 (< 3 kDa),它是 表明只有前两个包含 EPS 的部分具有容量 以降低 SFT,而 < 3 kDa 级分显示与以下相同的 SFT 纯海水或只有真正溶解有机碳的海水。


显微镜技术(即 CLSM 和 SEM)证实,正如预测的那样,蛋白质主要在空气 - 水界面富集, 强烈影响空气/水界面处的 SFT 治疗。 这些技术还可视化了不同的聚集体尺寸 和它们的分散,以及聚集体形成的重要性 通过阴离子EPS组分部分之间的Ca2+“桥接”。 SFT 可能会发生微小的变化,与蛋白质:多糖比率的变化相吻合,这可能是 pH 值变化的原因(十分之一) 单位),如 EPS 模型化合物所示,这可能在 CMC 周围最为突出。 此外,我们表明蛋白质和酸性多糖的 EPS 模型成分比 Corexit 导致海水中胶束的自组装甚至 当这些成分的浓度很低时。 这个 表明 EPS 在形成方面与 Corexit 相同或更有效 乳液。 然而,关于相互作用的更系统的研究 不同组件的不同组合,以及更多型号 单独的化合物,可能需要更多地阐明在我们的中宇宙实验中观察到的复杂性。


致谢


这项研究得到了墨西哥湾的资助 支持名为 ADDOMEx 的联盟研究的研究计划 (微生物对分散剂和油的聚集和降解 Exopolymers) 联盟。 原始数据可以在海湾找到 墨西哥研究倡议信息和数据合作组织 (GRIIDC) 在网址 https://doi.org/10.7266/N7PK0D64; https://doi.org/10。 7266/N78P5XZD; https://doi.org/10.7266/N74X568X; https://doi. org/10.7266/N79W0D1K。


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蛋白质外聚物中多糖的比例——摘要、简介

蛋白质外聚物中多糖的比例——方法

蛋白质外聚物中多糖的比例——结果与讨论

蛋白质外聚物中多糖的比例——结论、致谢!

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